Canine Distemper Test Strips - Tools For The Detection Of Canine Mutated Virus

Canine distemper test strip function: detect the mutated virus in the dog's serum, plasma, eye secretions and nasal mucus, and can detect 100% canine distemper.

Introduction

Canine distemper is a large, highly contagious, lethal infectious disease caused by canine distemper virus.

Early biphasic fever type with cold-like symptoms, followed by bronchitis, catarrhal pneumonia, and gastroenteritis.

In the later stage of the disease, neurological symptoms such as spasms and convulsions can be seen. In some cases, hyperkeratosis of the nose and angular pads (hardfoot) may occur.

Symptoms

The incubation period for canine distemper is 3-9 days. Symptoms vary, and are related to the strength of virulence, environmental conditions, age and immune status. Canine distemper begins with an elevated body temperature that lasts 1-3 days. Then it subsided, much like the characteristics of a cold recovery. But the temperature rose again after a few days for an indeterminate time. It can be seen that there are tears, redness of the conjunctiva, and eye secretions change from liquid to purulent. The nasal environment is dry, and there is nasal discharge, which is initially serous, and then becomes purulent. At the beginning of the disease, there was a dry cough, and later it turned into a wet cough and difficulty in breathing. Vomiting, diarrhea, intussusception, and eventually death with severe dehydration and debility.

Usage and Dosage

1. Collection and storage of the objects to be picked up: Plasma, serum, eye secretions and nasal mucus are used as the collection materials, and 100ul of the objects to be detected are required. The tested objects can be stored for 7 days at 2-8 degrees. If the test substance is stored for a long time, the serum or plasma should be separated and stored at -20°C. When carrying out the test, it should be fully placed in the room temperature environment, so that the temperature of the tested object is consistent with the room temperature before the test is carried out.

2. Detection method: The reaction plate is placed in a humid environment, which may cause the droplet concentration and stability of the antibody to decrease. Therefore, it should be opened and extracted before use. Remove the silver foil, place the tool on a flat surface, and record the relevant information of the sick animal on the tool. If the test object is serum or plasma, drop 2-3 drops (100ul) on the test object with a dropper. If it is eyeball secretions (tears) or nasal mucus, use a cotton swab for sampling to collect the test object and dilute it in a dilution buffer, then drop 2-3 drops on the test object compartment. After 5-10 minutes, judge the test result. After placing the test object, the test object is completely diffused on the reaction plate, and the judgment is made within 10 minutes, and the reaction after 10 minutes is regarded as negative.

3. Judgment results: according to the results of the test line and the control line to judge the negative and positive.

Negative: When only the control line (C) is red or purple. This condition indicates that there is no canine distemper infection.

Positive: Only when both the test line (T) and the control line (C) are red or purple. This situation indicates that the canine distemper virus has been infected.