Canine Distemper Test Paper-Canine Distemper Test Paper

Pet antigen test strips generally use double antibody sandwich immunogold chromatography technology, which is a one-step solid-phase membrane reaction. After the test paper is dropped into the sample solution, the antigen in the solution crawls to the binding pad with the solution, and reacts with the colloidal gold labeled antibody 1 in it to form a red antigen-antibody complex, and then continues the chromatography to move forward. At the detection line (T) of the observation window, the antigen in the red immune complex is captured and intercepted by the pre-coated antibody 2 here. As the interception of the red complex increases, a red line is gradually formed, so according to the red T line The appearance of the sample solution can be judged as positive. If there is no antigen in the sample, no immune antigen-antibody complex will be formed, and it will flow through the T area without trapping and trapping. The T area remains white, and it is judged as negative.

Sampling method

Canine distemper virus detection should take dog nose, eye secretions, saliva, serum, and anal gland secretions as test samples.

Detection method

After the test paper card is taken out from the aluminum foil bag, it is required to lay it flat on the table, and use a pipette to suck the sample solution into the sample hole and add 3 to 4 drops drop by drop. The reaction is generally completed within 5-20 minutes.

If the stool sample is turbid, it may block the sample flow at the sample hole. When the viscosity of some serum is too high, it will cause the chromatography to flow slowly. In this case, add 1-2 drops of diluent to promote the smooth flow of the sample. , To ensure that the red solution climbs up and flows through the reaction membrane smoothly, so that better reaction results can be obtained;

After sampling the stool, mix it with the diluent thoroughly, let it stand for 1 minute, and then take the supernatant. Note that the sampling solution should not contain feces and suspended impurities, it will block the pores in the sample hole, the solution is difficult to penetrate, and the solution cannot climb up the reaction membrane in the observation window, and the chromatography flows to the opposite side of the reaction membrane until The membrane surface returned from red to white, and the reaction line clearly appeared. Therefore, the reaction cannot proceed smoothly, and a white board phenomenon may occur.

Caochenyuan's precautions:

Before adding the sample, the reaction sample and the diluent should be thoroughly mixed.

Add sample liquid drop by drop (interval 2 seconds), 3-4 drops, until red liquid flows out from the display window.

When there is no red solution at the edge of the observation window after adding 3-4 drops of sample for 30 seconds, add 2 drops as appropriate.

In order to avoid the phenomenon of overflow of the solution and affecting the reaction signal, it is not advisable to fill the reaction well at one time.

Result judgment

The pet antigen detection test paper generally adopts the antibody sandwich method. Interpretation of Sandwich Test Paper Reaction Results

described as follows:

①Positive: There are purple-red bands at the quality control line (C) and detection line (T), which are judged to be positive;

②Negative: A purple-red band appears at the quality control line (C), and a purple-red band does not appear at the test line (T), it is judged as negative;

③Invalid: There is no red band on the quality control line (C), regardless of whether there is a red band on the T line, the test strip is judged to be invalid.