PRRSV Ab

Porcine Reproductive and Respiratory Syndrome Virus Antibody Rapid Test (PRRSV Ab)

Catalog No.:JIP505

¤  INTENDED USE

The Porcine Reproductive and Respiratory Syndrome Virus Antibody Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Porcine Reproductive and Respiratory Syndrome Virus Antibody (PRRSV Ab) in pig’s serum, or plasma specimen.

Assay Time:  5-10 minutes

Specimen: serum, plasma, or milk specimen.

¤ PRINCIPLE

The Porcine Reproductive and Respiratory Syndrome Virus Antibody Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated PRRSV antigens. If there are anti-PRRSV antibodies in the specimen, a visible T line will appears. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can indicate the presence of Porcine Reproductive and Respiratory Syndrome Virus antibodies in the specimen.

¤  TEST PROCEDURE

- Collect pig’s fresh whole blood, and get the serum or plasma specimen for use. Immediately apply the testing once collected the specimen.

- Take out the test device from the foil pouch and place it horizontally.

- Place 3 drops (approx. 120μL) of serum or plasma specimen into the assay sample hole “S”.

- Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.

¤  INTERPRETATION OF RESULTS

Negative: Only clear C band appear. No T band. It indicates that the titer of PRRSV antibody in the specimen is below 1:40.

Positive: The presence of both “C” line and zone “T” line. It indicates that the titer of PRRSV antibody in the specimen is above 1:40. The deeper the color, the higher the titer.  

Invalid: No colored band appears in C zone. No matter if T band appears.

¤  WHAT IS PRRSV?

The porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important swine diseases in the world. It is causing an enormous economic burden due to reproductive failure in sows and a complex respiratory syndrome in pigs of all ages, with mortality varying from 2 to 100% in the most extreme cases of emergent highly pathogenic strains. 

¤  WHAT IS THE CLINICAL SIGN?

PRRS is probably the most important swine disease of the last half-century. Serological testing has revealed there are many infected herds in which signs are not apparent. Where signs are apparent, they vary and are influenced by (1) virulence of the virus, (2) whether it is an initial infection or ongoing (endemic with herd immunity), (3) the age group affected, (4) other disease causing agents present in the population, and (5) herd size and management practices.

Breeding age gilts, sows, and boars: Clinical signs may include a period of anorexia, fever, lethargy, depression, and perhaps respiratory distress or vomiting. Mild cyanosis of the ears, abdomen and vulva has been reported in some outbreaks. Reproductive problems, often the most obvious signs, include a decrease in the number of dams that conceive or farrow. There is usually an increase in premature farrowings, late term abortions, stillborn or weak piglets and mummified fetuses. Preweaning mortality is high. Nursing pigs may have dyspnea (“thumping”). The period for reproductive signs varies with herd size but is usually two to three months in duration. A slow improvement in reproductive performance then begins. In larger operations, signs may be cyclical, especially if naïve gilts or sows continue to be introduced into the herd. There is evidence that subpopulations within large breeding herds escape initial infection but are infected when exposed later and serve as sources of continued virus shedding. Also, herds may be infected with multiple, heterologous strains of PRRS virus that are not completely cross-protective. In boars, clinical signs are similar to sows and are accompanied by a decrease in semen quality.

Young, growing and finishing pigs: Primary clinical signs among young pigs are fever, depression, lethargy, stunting due to systemic disease, and pneumonia. Sneezing, fever and lethargy are followed by expiratory dyspnea and stunting. Peak age for respiratory disease is four to ten weeks. Postweaning mortality often is markedly increased, especially with more virulent strains and the occurrence of ever-present concurrent and secondary infections. Older pigs, especially naïve, high-health swine, have similar respiratory signs. Heterologous infections may lead to prolonged or repeated outbreaks of respiratory disease.

J&G Biotech Ltd (Reg. No.: 08419172)

326 Cleveland Road, London, England E18 2AN, UK