O.Brucella Ab Rapid Test

Ovine Brucella Antibody Rapid Test (O.Brucella Ab)

Catalog No.:JIB601

INTENDED USE

The Ovine Brucella Antibody Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of ovine Brucella Antibody (B.Brucella Ab) in sheep’s serum, plasma, or milk specimen.

Assay Time:  5-10 minutes

Specimen: serum, plasma, or milk specimen.

PRINCIPLE

The Ovine Brucella Antibody Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated Brucella LPS antigens. If there are anti-Brucella antibodies in the specimen, a visible T line will appears. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Ovine Brucella antibodies in the specimen.

Brief Introduction

Ovine brucellosis is a bacterial disease caused by the gram-negative bacteria Brucella ovis. It primarily affects sheep and causes reproductive problems in both rams and ewes. The disease is endemic in many sheep rearing countries of the world including Australia, New Zealand, Turkey, Greece, Bulgaria, Romania, and Iran. Infected sheep pass the bacteria in their semen and infected rams can infect other ewes during breeding. The bacteria can also be spread through contaminated equipment, feed, and water.

Currently, the gold standard for diagnosing ovine brucellosis is the serum agglutination test (SAT), which detects antibodies against the bacteria in the serum of infected sheep. However, the SAT is time-consuming, requires specialized equipment and trained personnel, and is not suitable for on-site detection. Therefore, the development of a rapid diagnostic test for ovine brucellosis is highly desirable.

The Ovine Brucella Antibody Rapid Test (OBART) is one such test designed to detect antibodies against B. ovis in sheep serum. The OBART is a lateral flow immunoassay that uses gold nanoparticles conjugated to B. ovis antigen as the detector. The test strip has a test line coated with B. ovis antigen and a control line that confirms the validity of the test. When a sheep serum sample is applied to the sample well, it flows through the test strip and binds to the B. ovis antigen on the test line, producing a visible line. A positive result is indicated when both the test line and control line are visible, while a negative test has only a control line visible.

The OBART has shown promising results in detecting ovine brucellosis in several studies. A study conducted in Iran evaluated the accuracy of the OBART and found that it had a sensitivity of 94% and a specificity of 97%. In comparison, the SAT had a sensitivity of 100% and a specificity of 96%. Another study in Argentina evaluated the OBART on sheep farms and found that it had a sensitivity of 84% and a specificity of 100%. The study concluded that the OBART could be used as a complementary test to the SAT for the diagnosis of ovine brucellosis in field conditions.

In conclusion, the OBART is a rapid diagnostic test that shows promising results for the detection of antibodies against B. ovis in sheep serum. It has the potential to be used as a complementary test to the SAT for the diagnosis of ovine brucellosis in field conditions. However, further studies are needed to determine its diagnostic performance in different sheep populations and under different field conditions.