CSFV Ab Test

Classical Swine Fever Virus Antibody Rapid Test

 (CSFV Ab)

Catalog No.:JIP501

¤  INTENDED USE

The Classical Swine Fever Virus Antibody Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Classical Swine Fever Virus Antibody (CSFV Ab) in pig’s serum, or plasma specimen.

Assay Time:  5-10 minutes

Specimen: serum, plasma, or milk specimen.

¤  TEST PROCEDURE

- Collect pig’s fresh whole blood, and get the serum or plasma specimen for use. Immediately apply the testing once collected the specimen.

- Take out the test device from the foil pouch and place it horizontally.

- Place 3 drops (approx. 120μL) of serum or plasma specimen into the assay sample hole “S”.

- Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.

¤  INTERPRETATION OF RESULTS

¤  WHAT IS CSFV?

Classical swine fever virus (CSFV) is a high consequence pathogen. Infection with highly virulent CSFV isolates generally leads to death of infected animals, whereas isolates of moderate to low virulence induce a chronic disease. Classical swine fever (CSF) is a notifiable disease to the World Organization for Animal Health (OIE). Suspected cases of CSF are reported to competent (local) animal health authorities. Suspected cases are then confirmed, via laboratory testing, by officially authorized laboratories. Reporting to OIE is conducted by the national animal health authority.

¤  WHAT IS THE CLINICAL SIGN?

Acute form (more virulent strains and/or younger pigs)

• Fever
• Anorexia, lethargy
• Reddening/lesions of the skin
• Conjunctivitis
• Swollen lymph nodes
• Blue colouring of the ears, limbs, tail, snout
• Constipation followed by diarrhoea
• Occasional vomiting
• Laboured breathing, coughing
• Movement problems
• Birthing problems such as, mummification, stillbirth, abortion, birth of persistently infected litter
• Death occurs 5–25 days after onset of illness

Chronic form (less virulent strains or partially immune herds)

• Dullness, varied apetite, fever, diarrhoea for up to 1 month
• Stunted growth

• Apparent recovery with eventual relapse and death within about 3 months

¤  Bovine viral diarrhea virus (BVDV) and border disease virus (BDV) of sheep

These two diseases and classical swine fever (CSF) are closely related members of the family Togaviridae. Naturally occurring infections with BVDV and BDV have occurred in swine. Their major importance in swine is that they both induce antibodies that can lead to serologic misdiagnosis of CSF. This can cause confusion in countries trying to eradicate CSF or maintain a CSF-free status. This confusion can be avoided if specific laboratory methods of CSF diagnosis are used.

Clinically apparent outbreaks of BVDV and BDV in swine are uncommon. Outbreaks that have been suspected are manifested in breeding herds as reproductive problems, such as poor conception rates, small litters, abortions, and an excessive number of dead and mummified fetuses. In infected, live piglets, the signs are expected to be similar to those of congenital CSF including deaths in piglets less than five weeks old, anemia, unthriftiness, congenital tremors and convulsions.

J&G Biotech Ltd (Reg. No.: 08419172)

326 Cleveland Road, London, England E18 2AN, UK