BTB Ab Combo Rapid Test

Bovine Tuberculosis Antibody Rapid Test

(BTB Ab)

Catalog No.: JIB302

¤  INTENDED USE

The Bovine Tuberculosis Antibody Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Bovine Tuberculosis Antibody (BTB Ab) in cattle’s serum, plasma. MPB70 and MPB83 chimera recombinant was applied in this assay.  

Assay Time:  5-10 minutes

Specimen: serum, plasma.

¤  PRINCIPLE

The Bovine Tuberculosis Antibody Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated Tuberculosis antigens. If there are anti-TB antibodies in the specimen, a visible T line will appears. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Bovine Tuberculosis antibodies in the specimen.

¤  REAGENTS AND MATERIALS

- 20 Test devices

- Disposable droppers

- 1 bottle of Assay Buffer

- 1 Products Manual

¤  STORAGE AND STABILITY

The kit can be stored at room temperature (4-30°C). The test kit is stable through the expiration date (18 months) marked on the package label. DO NOT FREEZE. Do not store the test kit in direct sunlight.

¤  TEST PROCEDURE

- Collect cattle's fresh whole blood, and get the serum or plasma specimen for use. Immediately apply the testing once collected the specimen.

- Take out the test device from the foil pouch and place it horizontally.

- Place 1 drop (around 30uL) of serum or plasma specimen into the sample hole. Then place 1 drop (around 30uL) of assay buffer into the sample hole immediately.

- Interpret the result in 5-10 minutes. Result after 15 minutes is considered as invalid.

¤  INTERPRETATION OF RESULTS

- Positive (+): The presence of both “C” line and zone “T” line, no matter T line is clear or vague.

- Negative (-): Only clear C line appear. No T line.

- Invalid: No colored line appears in C zone. No matter if T line appears.

¤  PRECAUTIONS

- All reagents must be at room temperature before running the assay.

- Do not remove test cassette from its pouch until immediately before use.

- Do not use the test beyond its expiration date. 

- The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.

- All specimens are of potential infection. It must be strictly treated according to the rules and regulations by local states.

¤  LIMITATION

Bovine Tuberculosis Antibody Rapid Test is for in vitro veterinary diagnosis use only. All result should be considered with other clinical information available with veterinarian. It is suggested to apply a further confirmative method when positive result was observed.

J&G Biotech Ltd (Reg. No.: 08419172)

326 Cleveland Road, London, England E18 2AN, UK